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. 2023 May 18;24(10):8952. doi: 10.3390/ijms24108952

Figure 8.

Figure 8

Evaluation of EMT process in the colonic mucosa of DSS-treated mice. (A) Immunohistochemistry and quantification for vimentin (brown). Nuclei are counterstained with hematoxylin. Original magnification 10×, scale bar 100 μm. (B) Quantitative Real Time PCR for vimentin (vim) expression. (C) Immunofluorescence and semi-quantitative analyses for E-cadherin (red). Nuclei are counterstained with DAPI (blue); Original magnification 20×, scale bar 50 μm. (D) Quantitative Real-Time PCR for e-cadherin (ecadh1) expression. (E) Immunofluorescence and semi-quantitative analyses for beta-catenin (red) and nuclear translation (purple, pink arrows), Nuclei are counterstained with DAPI (blue). These images are representative of at least n = 3 animals/groups. Data were analyzed by ANOVA (vimentin, p = 0.0146; vim, p = 0.0103; E-cad, p = 0.0006; ecadh1, p < 0.0001; nuclear β-cat, p = 0.0012) using Tukey’s test for multiple comparisons (indicated in graphs). Ctl, control; DSS, DSS treated; GED, DSS treated plus GED; ASA, DSS treated plus 5-ASA.