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Inp52p and Inp53p expressed under the control of their native promoters localize to actin patches following hyperosmotic stress. Cells expressing Inp52p-GFP or Inp53p-GFP under the control of the respective native 5-phosphatase promoter were treated with 0.9 M NaCl for 10 min, formaldehyde fixed, stained with phalloidin, and then analyzed by confocal microscopy. Bar, 5 μm.
