FIG. 7.

Analysis of the domains mediating translocation of Inp53p to actin patches following hyperosmotic stress. Inp53p-GFP deletion mutant constructs were prepared as described in Materials and Methods. (A) Schematic diagram of the Inp53p mutant constructs. 5-ptase, 5-phosphatase; proline, proline-rich region. (B) Cells expressing Inp53p-GFP mutants (middle panels) were analyzed by confocal microscopy following 10 min of 0.9 M NaCl stress, fixed, and stained with phalloidin (right panels). Bar, 5 μm.