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Primers and corresponding assay for diagnosis of S. glycines on soybean.
| Target Gene | Primer/Probe (Combination) | Sequence (5′-3′) | Tm (°C) | Fragment Length (bp) | Assay | Reference |
|---|---|---|---|---|---|---|
| ACT | Ac1(f) | ACAATCCAGGGACCACAATC | 60 a | 90–100 | qPCR | [118] |
| Ac2(r) | ATGGCTGATCGCATACCC | 59 a | ||||
| Ac(probe) | 6FAM-AGAGCTGACCAGGACCCAGCATCCA-TAM | 73 a |
a Calculated primer melting temperatures. The assay was run with an annealing temperature of 60 °C.
