Table 8.
Primers and corresponding assays for diagnosis of M. phaseolia.
| Target Gene | Primer/Probe (Combination) | Sequence (5′-3′) | Tm (°C) | Fragment Length (bp) | Assay | Ref. |
|---|---|---|---|---|---|---|
| ITS | MpKFI | CCGCCAGAGGACTATCAAAC | 56 | 350 | PCR | [125] |
| MpKRI | CGTCCGAAGCGAGGTGTATT | |||||
| MpKH1 a | GCTCTGCTTGGTATTGGGC | 55 a | dot blot | |||
| Ukn b | MpSyK F | ATCCTGTCGGACTGTTCCAG | 60 | qPCR | [126] | |
| MpSyK R | CTGTCGGAGAAACCGAAGAC | |||||
| MpTqK F | GCCTTACAAGGGTCTCGTCAT | 60 | qPCR | |||
| MpTqK R | CCCTTGGCGATGCCGATA | |||||
| MpTqK P | 6-FAM-CAGGCCACAGGATCTT-MGBNFQ | |||||
| ITS | F3 | GCACATTGCGCCCCTTG | 62 c | LAMP | [127] | |
| B3 | GTTCAGAAGGTTCGTCCGG | |||||
| FIP | AGGACGGTGCCCAATACCAAGCGGGGCATGCCTGTTCGA | |||||
| BIP | CTCAAAGACCTCGGCGGTGGGCTCCGAAGCGAGGTGTA |
a DIG labeled probe. Correspondingly also the temperature is the hybridization temperature. b Sequence obtained using the SCAR approach. c Incubation for the LAMP assay. Incubation for 60 min.