Table 11.
Target Gene | Target Species (Specificity) | Primer/Probe (Combination) | Sequence (5′-3′) | Tm (°C) | Fragment Length (bp) | Assay | Ref. |
---|---|---|---|---|---|---|---|
ITS | P. pachyrhizi | Ppm1 | GCAGAATTCAGTGAATCATCAAG | 65 a 60 b | 141 | PCR/qPCR | [150] |
Ppa2 | GCAACACTCAAAATCCAACAAT | ||||||
ITS | P. meibomiae | Ppm1 | GCAGAATTCAGTGAATCATCAAG | 139 | |||
Pme2 | GCACTCAAAATCCAACATGC | ||||||
ITS | Phakopsora | Ppm1 | GCAGAATTCAGTGAATCATCAAG | 77 | |||
Ppm2 | CTCAAACAGGTGTACCTTTTGG | ||||||
ITS | Phakopsora | FAM-probe c | FAM-CCAAAAGGTACACCTGTTTGAGTGTCA-TAMRA | ||||
VIC-probe d | VIC-TGAACGCACCTTGCACCTTTTGGT-TAMRA | ||||||
ITS | P. pachyrhizi | ITS1rustF4a e | GAGGAAGTAAAAGTCGTAACAAGGTTTC | 60 | nested qPCR | [151,152] | |
ITS1rustF10d | TGAACCTGCAGAAGGATCATTA | ||||||
ITS1rustR3d | TGTGAGAGCCTAGAGATCCATTG | ||||||
ITS1PhpFAM1 | FAM-TCATTGAT-TGATAAGATCTTTGGGCAATGG-3IABlkFQ |
a With standard PCR. b With qPCR. c For use with Ppm1/Ppa2 and Ppm1/Pme2. d For use with Ppm1/Ppm2. e Combined with Ppa2 from [150] in first round of nested PCR.