Table 13.
Primers and corresponding assays for diagnosis of Pythium spp. on soybean.
| Target Gene | Target Species (Specificity) | Primer/Probe (Combination) | Sequence (5′-3′) | Tm (°C) | Fragment Length (bp) | Assay | Ref. |
|---|---|---|---|---|---|---|---|
| ITS | P. ultimum and HS group | K1 | ACGAAGGTTGGTCTGTTG | 55 | PCR | [175] | |
| K3 | TCTCTACGCAACTAAATGC | ||||||
| ITS | P. aphanidermatum | Pa1 | TCCACGTGAACCGTTGAAATC | 67 72 |
210 150 |
PCR | [176] |
| none | ITS2 | GCTGCGTTCTTCATCGATGC | |||||
| P. irregulare | Pir1 | AGCGGCGGGTGCTGTTGCAG | |||||
| ITS | P. aphanidermatum | AsAPH2B | GCGCGTTGTTCACAATAAATTGC | 57 a | 163 150 |
PCR | [177] |
| Pythium | AsPyF | CTGTTCTTTCCTTGAGGTG | 52 a | ||||
| P. torulosum | AsTOR6 | CGCCTGCCGAAACAGACTAG | 59 a | ||||
| Gene encoding a spore cell wall protein | P. ultimum | F3 | CAACTGGAAAAGCAAGCGG | 64 b | LAMP | [178] | |
| B3 | CCGAAGAACTGTGTCCGC | ||||||
| FIP | GAGCCAGACGGGCCAGTATCAAGTTACAGTGGCGTTGTCA | ||||||
| BIP | TCTCTGTTGCTCGACTGGAGGGTTCCACCTCCTGTAAGACCT | ||||||
| F-Loop | GCTTGCTCCAGTACGAATGC |
a Calculated melting temperatures. The general Pythium primer AsPyF can be combined either with AsAPH2B or AsTOR6. b Incubation for the LAMP assay. Incubation for 60 min.