Table 15.
Targets and primers for soybean as reference for quantification relative to soybean and other internal controls.
| Target | Primer | Sequence (5′-3′) | Tm (°C) | Fragment Length (bp) | Reference |
|---|---|---|---|---|---|
| cox1 | FMPI2b | GCGTGGACCTGGAATGACTA | 57 | [166] | |
| FMPI3b | AGGTTGTATTAAAGTTTCGATCG | ||||
| Plant-IC probe | CalFluorRed610-CTTTTATTATCACTTCCGGTACTGGCAGG-BHQ-2 | ||||
| cox1 | Cox1-IPC-F | CATGCGTGGACCTGGAATGACTATGCATAGA | 39 a | [168] | |
| Cox1-IPC-R | GGTTGTATTAAAGTTTCGATCGGTTAATAACA | ||||
| Cox1-IPC-P | GGTCCGTTCTAGTGACAGCATTCCYACTTTTATTA [TAM-dT] C [THF] C [BHQ2-dT] YCCGGTACTGGC [3′-C3SPACER] | ||||
| GAPDH | GmG-14F | CATCGGAGGGAAGTATGAAAGG | [187] | ||
| GmG-14R | GTACAATGCATGATGGTGGC | ||||
| GmG-14HEX | HEX-TTTGTGGGTGACAACAGGTGATGG-IBFQ | ||||
| HHIC | HHIC-Fwd | CTAGGACGAG AACTCCCACA T | 111 | [187] | |
| HHIC-Rev | CAATCAGCGG GTGTTTCA | ||||
| HHIC-HEX | HEX-TCGGTGTTGA TGTTTGCCAT GGT-IBFQ | ||||
| HHIC b | CACGCCTAGG ACGAGAACTC CCACATCGAG CTTGACGCAA ACGACCACGC CAGGACCATG GCAAACATCA ACACCGAGCG CAACGCCTTG TGCTGAAACA CCCGCTGATT G b |
a Incubation for the RPA assay. Incubation for 29 min; for details see [169]. b Sequence of the artificial internal control target HHIC (Haudenshield and Hartman internal control).