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. Author manuscript; available in PMC: 2023 Nov 3.
Published in final edited form as: Nature. 2023 May 3;617(7960):377–385. doi: 10.1038/s41586-023-06026-3

Extended Data Fig 10. Gating strategies for flow cytometric analysis.

Extended Data Fig 10.

The following gating schemes were used to define CD11c+ MHC II+, CD11b+ MHC II+ cells, CD8+ T cells, CD4+ T cells and Treg cells in (a) draining lymph nodes and mesenteric lymph nodes and (b) tumor-infiltrating immune cells. (c) Gating strategy to examine OT-I cells stimulated by OVA-loaded BMDCs. (d) Gating strategy used to analyze tumor cells from the mice implanted with MC38 tumor cells expressing GFP. Gating strategies used to sort CD4+ T cells, CD8+ T cells, CD11c+MHC II+ cells and CD11b+ cells from (e) tumor-infiltrating leukocytes and (f) tumor draining lymph nodes.