Table 3.
List of used antibodies.
| Antibodies | Company | Cat. Ref. | Raised/Type | Dilution |
|---|---|---|---|---|
| Primary antibodies | ||||
| Anti-TH | Millipore, Burlington, VT, USA | AB152 | IgG rabbit polyclonal | 1:400 |
| Anti-LMX1A | Abcam, Cambridge, UK | ab139726 | IgG rabbit polyclonal | 1:100 |
| Anti-TUJ1 | Covance, Princeton, NJ, USA | MMS-435P | IgG2a mouse monoclonal | 1:1000 |
| Anti-GBA | Abcam, Cambridge, UK | Ab125065 | IgG rabbit monoclonal | 1:500 |
| Anti-GAPDH | Sigma-Aldrich, Darmstadt, Germany | SAB2108266 | IgG rabbit polyclonal | 1:18000 |
| Secondary antibodies | ||||
| Alexa Fluor 488 goat anti rabbit IgG (H + L) | Thermo Fisher Scientific, Waltham, MA, USA | A11008 | Goat | 1:400 |
| Alexa Fluor 568 goat anti rabbit IgG (H + L) | Thermo Fisher Scientific, Waltham, MA, USA | A11011 | Goat | 1:400 |
| Alexa Fluor 488 goat anti mouse IgG2a | Thermo Fisher Scientific, Waltham, MA, USA | A21131 | Goat | 1:400 |
| Goat Anti-Rabbit IgG H&L (HRP) | Abcam, Cambridge, UK | Ab6721 | Goat | 1:5000 |