Fig. 2. Collagen cross-linking and Lox/Loxl expression in the bleomycin model.
(A) Schematic regime of Rosa26Cre-ERT2 mice under intratracheal (IT) bleomycin challenge. Lungs were harvested for terminal analyses on day 24 after bleomycin challenge. (B to F) mRNA expression of Lox family members from lung tissue. (G) Lung total OHP. (H to K) Quantification of lung collagen cross-linking by (H) total DHLNL, (I) DHLNL normalized by collagen, (J) total PYD, and (K) PYD normalized by collagen. Saline, n = 6; bleomycin, n = 16. Data represent means ± SD. *P < 0.05, **P < 0.01, and ****P < 0.0001. P value is calculated using unpaired, two-tailed t test (B) to (K).