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. 2023 May 26;9(21):eadf0133. doi: 10.1126/sciadv.adf0133

Fig. 5. LOXL4 deficiency disrupts TGFβ signaling and inactivates myofibroblasts in the bleomycin injured lungs.

Fig. 5.

(A and B) (A) Representative immunofluorescent images of ACTA2 in lung tissue with quantification of (B) percentage of ACTA2+ area. (C and D) mRNA expression of selective (C) YAP/TAZ target genes and (D) TGFβ target genes from lung tissue. (E and F) (E) Representative immunofluorescent images of TAZ and ACTA2 in lung tissue with quantification of (F) number of TAZ+ nuclei/ACTA2+ cells per field. (G and H) (G) Representative immunofluorescent images of YAP and ACTA2 in lung tissue with quantification of (H) number of YAP+ nuclei/ACTA2+ cells per field. Arrowheads present (E) TAZ+ nuclei/ACTA2+ cells and (G) YAP+ nuclei/ACTA2+ cells. (I and J) (I) Representative immunofluorescent images of pSMAD3 and ACTA2 in lung tissue with quantification of (J) number of pSMAD3+/ACTA2+ cells per field. Data in (B), (F), (H), and (J) represent mean value of five random fields per image. Data represent means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. P value is calculated using one-way ANOVA.