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. 2023 May 26;9(21):eadf6232. doi: 10.1126/sciadv.adf6232

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Fig. 4. — The schematic presentation of the experiment is shown in the top left corner. HS polysaccharide (from wild-type Chinese hamster ovary cells) was individually incubated with five different 3-O-sulfotransferase (3-OST) isoforms. The amount of 3-O-sulfated tetrasaccharides was determined by the LC-MS/MS. Each data point is the average of three independent determinations ± SD. mIU, milli-international units.