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The IIE phenotype of wild-type (RHΔ) and two Iie− mutants (MBE1.1 and MBE3.3). (a) A time course of egress using 1 μM A23187 in HBSSc. (b) IIE as a function of A23187 concentration. Egress was measured after 2 min of exposure to the ionophore. Monolayers for both panels a and b were scored for the percentage of lysed vacuoles per field.
