FIG. 5.
The effect on EOP of pretreating extracellular parasites with either A23187 or BAPTA-AM. Extracellular parasites were pretreated with either 1 μM A23187 or 50 μM BAPTA-AM in HBSSc for 30 min at 37°C and were plated to determine the EOP expressed as the percent relative to treatment with DMSO alone. The data are pooled from three independent trials. The two Iid+ strains (RHΔ and MBE3.3) showed the expected drop in EOP after exposure to A23187. Upon treatment with BAPTA-AM, RHΔ, MBE1.1, and MBE3.3 showed about a 90% drop in EOP while MBD2.1 was hypersensitive to this treatment, with a drop of over 99%.