Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus, male and female) | Atf3tm1.1(cre/ERT2)Msra (Atf3CreERT2) | Matt Ramer | MGI: 6360893 | PMID:26610346 |
| Strain, strain background (Mus musculus, male and female) | C57BL/6-Tg(Cdh5-cre/ERT2)1Rha (Cdh5CreERT2) | Taconic | 13073, MGI: 3848982 | PMID:19144989 |
| Strain, strain background (Mus musculus, male and female) | Gt(ROSA)26Sortm14(CAG-tdTomato)Hze (ROSA26LSL-tdTomato) | Jackson Laboratory | 007914, MGI: 3809524 | PMID:20023653 |
| Strain, strain background (Mus musculus, male and female) | Atf3tm1.1Hai (Atf3lox) | Tsonwin Hai | MGI: 5547956 | PMID:23921126 |
| Antibody | tdTomato (goat polyclonal) | Origene | AB8181-200 | 1:50 |
| Antibody | RFP (DsRed) (rabbit polyclonal) | Rockland | 600-401-379, RRID:AB_2209751 | 1:50 |
| Antibody | ERG (mouse monoclonal) | Abcam | ab214341 | 1:100 |
| Antibody | ERG (rabbit monoclonal) | Abcam | ab92513 RRID:AB_2630401 |
1:100 |
| Antibody | Endomucin-1 (goat polyclonal) | R&D Systems | AF4666 RRID:AB_2100035 | 1:500 |
| Antibody | LAMP3 (rat monoclonal) | Novus Biologicals | DDX0191P-100 RRID:AB_2827532 | 1:100 |
| Antibody | HOPX (mouse monoclonal) | Santa Cruz Biotechnology | sc-398703 RRID:AB_2687966 | 1:100 |
| Antibody | NKX2-1 (TTF1) (rabbit monoclonal) | Abcam | ab76013 RRID:AB_1310784 | 1:50 |
| Antibody | PDPN (mouse monoclonal) | Abcam | ab10288 RRID:AB_297027 | 1:100 |
| Antibody | CD45-PerCP-Cy5.5 (rat monoclonal) | Thermo Fisher | 45-0451-82 RRID:AB_1107002 | 1:200 |
| Antibody | CD31-PE-Cy7 (rat monoclonal) | Thermo Fisher | 25-0311-82 RRID:AB_2716949 | 1:100 |
| Chemical compound, drug | PR8-GP33 H1N1 influenza | Dr. E. John Wherry | PMID:23516357 | |
| Chemical compound, drug | Tamoxifen | Sigma-Aldrich | T5648 | |
| Commercial assay or kit | Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit | Thermo Fisher | C10632 | |
| Commercial assay or kit | RNAscope Fluorescent Multiplex Reagent Kit v2 | ACD | 323100 | |
| Commercial assay or kit | PureLink RNA Micro Kit | Thermo Fisher | 12183–016 | |
| Commercial assay or kit | Bioanalyzer High Sensitivity RNA Pico Kit | Agilent | 5067–1513 | |
| Commercial assay or kit | NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina | New England Biolabs | E6420 | |
| Commercial assay or kit | Bioanalyzer High Sensitivity DNA Kit | Agilent | 5067–4626 | |
| Other | RNAscope probe mm-Car4-C3 | ACD | 468421-C3 | Probe to detect Car4 RNA; see Figure 5—figure supplement 3 and Materials and methods: RNAscope analysis |
| Other | RNAscope probe mm-Pdgfra-C2 | ACD | 480661-C2 | Probe to detect Pdgfra RNA; see Figure 2—figure supplement 2 and Materials and methods: RNAscope analysis |