Fig. 1.

Effect of hyperthermia on breast cancer cells. A Normal breast cells (MCF-10 A), Luminal type human breast cancer cells (MCF-7, T47D), Her2 positive human breast cancer cells (SKBR-3) and TNBC cells (MDA-MB-231, Hs578T) were treated with 43 °C water bath for 1 h. Cell viability was measured by using the CCK-8 assay. B Cell apoptosis analysis of MDA-MB-231 and Hs578T cells treated with or without hyperthermia were performed using flow cytometry assay. C Cell cycle of MDA-MB-231 and Hs578T cells were analyzed using flow cytometry assay before and after hyperthermia treatment. D The statistical data of cell apoptosis and cell cycle using GraphPad Prism 8.0