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. 2023 May 26;14:81. doi: 10.1007/s12672-023-00697-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Exosomes secreted by hyperthermia TNBC cells can promote M1 macrophage differentiation. A Uptake analysis of exosome by macrophage cells and breast cancer MCF-7 cells by confocal microscope, Scale bar = 50 μm. B Taking LPS (100 ng/ml)-induced M1 type macrophages as positive control, the expression levels of M1 markers (IL-12 and iNOS) in macrophages co-cultured with exosomes were tested by RT-qPCR. C The expression levels of M2 markers (CD206 and ARG1) in macrophages co-cultured with exosomes were tested by RT-qPCR. D The proportions of CD14 + CD86 + M1 macrophages and CD14 + CD163 + M2 macrophages were analyzed by flow cytometry