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. 2023 May 26;14:81. doi: 10.1007/s12672-023-00697-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Exosome-mediated HSPB8 released by TNBC cells under hyperthermia modulates polarization in macrophages. A The expression of HSPA1A, HSPA1B, HSPA6 and HSPB8 in exosomes released by MDA-MB-231 after hyperthermia. B The transfection efficiency of HSPB8 OE plasmids were tested by RT-qPCR. C The expression levels of M1 markers (IL-12 and iNOS) and M2 markers (CD206 and ARG1) in macrophages transfected with HSPB8-OE were tested by RT-qPCR. D Detection of CD86 expression in macrophages transfected with HSPB8-OE by immunofluorescence assay, Scale bar = 100 μm. E The proportions of CD86 + M1 TAM and CD163 + M2 TAM were analyzed by flow cytometry