Fig. 1.

Complementary methods for detecting genetic variants associated with T1D. (a) The effect size of any given variant on T1D risk is inversely related to the frequency of the variant⁵. (b) To detect more commonly observed variants, DNA samples from nPOD donors were probed for the presence of SNPs previously reported in T1D GWAS² efforts using the UFDIchip, yielding CEL microarray image files (modified from Affymetrix Axiom website: https://www.affymetrix.com/products_services/arrays/specific/axiom_mydesign.affx). (c) To detect rare or novel variants, whole exome sequencing (WES) was performed on DNA from nPOD donors (modified from Roche NimbleGen SeqCap EZ Exome Library workflow⁶⁸). Diagrams created in BioRender.