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. 2023 May 26;14(5):342. doi: 10.1038/s41419-023-05864-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Representative blots for indicated proteins of scramble (control) siRNA, Beclin1 siRNA and hCG-treated luteinized granulosa cells. Densitometric quantification is indicated to the right of the blots. Mean ± SD, N = 5 biological replicates analyzed using one-way ANOVA, with Tukey’s test for multiple comparisons. B Representative graphic bar indicates progesterone (P₄) production of the luteinized granulosa cells transfected with control siRNA, Beclin1 siRNA and treated with hCG (10 IU/ml). Mean ± SD, N = 5 biological replicates analyzed using one-way ANOVA, with Tukey’s test for multiple comparisons. C Representative blots for indicated proteins of control (scramble) siRNA and Atg5 siRNA-treated luteinized granulosa cells. Densitometric quantification is indicated to the right of the blots. Mean ± SD, N = 3 biological replicates analyzed using one-way ANOVA, with Tukey’s test for multiple comparisons. D Representative graphic bars indicate progesterone (P₄) production of the luteinized granulosa cells transfected with control siRNA, Atg5 siRNA and treated with hCG (10 IU/ml). Mean ± SD, N = 3 biological replicates analyzed using one-way ANOVA, with Tukey’s test for multiple comparisons. E Representative blots for indicated proteins of control (FF) shRNA, Beclin1 shRNA and FSH-treated non-luteinized granulosa cells (HGrC1). Densitometric quantification is indicated to the right of the blots. Mean ± SD, N = 3 biological replicates analyzed using one-way ANOVA, with Tukey’s test for multiple comparisons. F Representative graphic bars indicate estradiol (E₂) production of the HGrC1 cells transfected with control shRNA, Beclin1 shRNA and treated with FSH at indicated concentrations. Mean ± SD, N = 3 replicates were analyzed using one-way ANOVA, with Tukey’s test for multiple comparisons.