Fig. 4.

A) pHSP27 immunoblot analysis on hCM + PBS, hCM + Pre_EVs and hCM + Post_EVs. B) Representative images of hCM + PBS, hCM + Pre_EVs, hCM + Post_EVs and Ctrl+ (hCM treated with H2O2) immunostained for NRF2 (red), cardiac-troponin T (cTnT-green) and DAPI; On the right panel, quantification of NRF2 nuclear translocation. Statistical significance were determined using one-way anova (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)