Fig. 5.

Hit triage. A) GPX1 and GPX4 HTS triage schematic; B) Summary of novel small molecule GPX1 actives in primary assay and counter-screens; C) Summary of novel small molecule GPX4 actives in primary assay and counter-screens; D) Heat map showing relationships of hit compounds based on the similarities of their activity profiles against GPX1, GPX2, and GPX4. For further details on each compound, see Table 2. Compounds 20 and 21 were not tested with GPX2 (shown as black box). Data is given as mean remaining activity at top concentration of n = 3 replicates.