Table 5.

Fatty acids profile of the standard feed, PHENOFEED DRY and enriched feed¹.

N.	Fatty acid	Items	Standard Feed (g/100 g)	PHENOFEED DRY (g/100 g)	Enriched Feed (g/100g)	SEM	Total intake due to 500 mg/day of PHENOFEED DRY	Total content (g) in 15 kg of enriched feed administered cow/day
1	Myristic	C14:0	3.838	2.983	3.839	0.10	0.015	575.70
2	Palmitic	C16:0	13.999***	12.971***	13.011***	0.29	0.065	2099.85
3	Trans-palmitoleic	C16:1n 7t	3.756	4.946	3.701	0.09	0.025	563.40
4	Palmitoleic	C16:1n 7	2.896	1.843	2.897	0.03	0.009	434.40
5	Stearic	C18:0	6.386	6.918	6.387	0.10	0.035	957.90
6	Oleic	C18:1 n9	19.111***	16.945***	19.111***	0.50	0.085	2866.65
7	Linolelaidic	C18:2 n6t	5.243	5.213	6.242	0.09	0.026	786.45
8	Linoleic	C18:2 n6	19.5***	15.339***	19.529***	0.36	0.077	2925.00
9	Gamma-linoleic	C18:3 n6	0.126	2.189	0.126	0.18	0.011	18.90
10	Eicosenoic	C20:1 n9	0.244	2.507	0.244	0.03	0.013	36.60
11	Alpha-linolenic	C18:3 n3	3.136	3.158	3.136	0.05	0.016	470.40
12	Eicosadienoic	C20:2 n6	0.146	1.621	0.146	0.03	0.008	21.90
13	Dihomo-γ-linolenic	C20:3 n6	0.396	3.118	0.403	0.05	0.016	59.40
14	Arachidonic	C20:4 n6	6.881	5.995	6.882	0.09	0.030	1032.15
15	Lignoceric	C24:0	0.194	1.179	0.194	0.10	0.006	29.10
16	Eicosapentaenoic	C20:5 n3	2.782	2.333	2.783	0.05	0.012	417.30
	(EPA)
17	Nervonic	C24:1 n9	3.673	3.511	3.675	0.05	0.018	550.95
18	Docosahexaenoic	C22:6 n3	7.693	7.231	7.694	0.14	0.036	1153.95
	(DHA)
	Total
		SFA	24.417	24.051	23.431	0.58
		MUFA	29.68	29.752	29.628	0.69
		PUFA	45.903*	46.197*	46.941*	1.04

¹Two-way Anova Analysis in standard feed, PHENOFEED DRY and enriched feed C16:0 vs C14:0 and C18:0 *** (p<0.001), C18:1 n9 vs C16:1 n7 *** (p<0.001), C18:2 n6 vs other PUFAs *** (p<0.001), PUFA vs MUFA and SFA * (p<0.05). SFA, saturated fatty acids; MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids. SEM: standard error of the mean.