. 2023 May 23;21:100298. doi: 10.1016/j.vas.2023.100298

Table 6.

Fatty acid composition in plasma of lactating Holstein-Friesian cows (mid-lactation) fed with a standard diet (T0) and diet supplemented with PHENOFEED DRY¹.

Fatty Acid (%)	Experimental Time																		SEM
	T0			T1			T2			T3			T4			T5
	P	S	PL	P	S	PL	P	S	PL	P	S	PL	P	S	PL	P	S	PL
C14:0	0.17	0.18	0.23	0.30	0.21	0.16	0.12	0.10	0.19	0.15	0.17	0.15	0.18	0.42	0.57	1.12	1.17	1.97	0.06
C16:0	12.80	13.29	12.05	11.21	10.50	10.54	10.38	8.73	9.98	9.25	10.29	10.03	9.31	9.59	10.12	12.80	14.65	14.58	0.33
C16:1 n7t	0.44	0.40	0.55	0.64	0.25	0.43	0.12	0.45	0.18	0.19	0.34	0.84	0.11	0.55	0.57	0.09	0.06	0.02	0.06
C16:1 n7	0.30	0.47	0.58	0.74	0.61	0.94	0.69	0.67	0.36	1.02	1.23	1.19	0.87	0.99	0.89	0.03	0.01	0.07	0.12
C18:0	18.55	18.77	19.85	16.98	21.20	19.34	16.40	13.08	12.92	15.56	13.24	16.58	13.20	15.18	14.72	21.91	23.69	24.90	0.68
C18:1 n9	10.01	11.70	11.56	12.55	13.64	13.16	14.44	14.85	19.59	14.30	19.09	17.15	16.63	16.77	17.58	22.43***	17.49	18.99	0.50
C18:1 n9	10.01	11.70	11.56	12.55	13.64	13.16	14.44	14.85	***	14.30	19.09	17.15	16.63	16.77	17.58	22.43***	17.49	18.99	0.50
C18:2 n6t	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
C18:2 n6	24.92	24.88	22.88	29.54	25.70	24.96	30.91	34.81	29.00**	26.56	23.18	22.98	28.61	26.16	24.94	36.66***	37.77***	34.58	0.71
C18:3 n6	0.48	0.47	0.35	0.77	0.46	0.34	0.75	0.78	0.38	0.71	0.46	0.57	0.65	0.36	0.37	0.31	0.22	0.20	0.06
C20:1 n9	0.86	0.44	0.50	1.42	1.00	1.00	1.31	1.20	1.19	1.87	1.93	1.44	1.24	0.59	0.29	0.53	0.31	0.12	0.14
C18:3 n3	0.48	0.45	0.65	0.35	0.26	0.32	0.33	0.29	0.22	0.54	0.49	0.35	0.38	0.32	0.36	0.25	0.29	0.31	0.07
C20:2 n6	0.20	0.16	0.10	0.10	0.04	0.13	0.16	0.36	0.75	0.97	0.42	0.04	0.56	0.77	0.20	0.19	0.28	0.11	0.05
C20:3 n6	3.66	3.49	3.96	4.13	4.70	5.21	4.21	4.73	4.55	6.50	6.67	5.51	6.10	4.41	5.20	0.41	0.42	0.82	0.21
C20:4 n6	4.45	4.85	4.72	6.10	5.98	6.90	5.39	5.00	5.82	6.85	6.28	6.08	5.50	5.77	6.06	1.85	1.97	1.82	0.15
C24:0	0.83	0.82	0.99	0.22	0.20	0.55	0.26	0.06	0.09	0.23	0.09	0.10	0.09	0.15	0.17	0.26	0.27	0.13	0.05
C20:5 n3	0.48	0.37	0.40	0.75	0.72	0.53	0.77	0.80	0.80	0.90	0.90	0.61	0.85	0.75	0.27	0.31	0.19	0.20	0.09
C24:1 n9	8.49	5.91	7.42	1.09	1.00	1.94	0.73	0.99	0.64	0.75	0.68	0.68	0.69	0.34	1.08	0.39	0.55	0.53	0.17
C22:6 n3	12.88	13.35	13.21	13.11	13.53	13.55	13.03	13.10	13.34	13.65	14.54	15.70	15.03	16.88	16.61	0.46	0.66	0.65	0.47
Total
SFA	32.35	33.06	33.12	28.71	32.11	30.59	27.16	21.97	23.18	25.19	23.79	26.86	22.78	25.34	25.58	36.09	39.78	41.58	1.12
SFA	32.35	33.06	**	28.71	32.11	**	27.16	21.97	**	25.19	23.79	**	22.78	25.34	**	36.09	39.78	**	1.12
MUFA	20.10	18.92	20.61	16.44	16.5	17.47	17.29	18.16	21.96	18.13	23.27	21.3	19.54	19.24	20.41	23.47	18.42	19.73	0.98
PUFA	47.55	48.02	46.27	54.85	51.39	51.94	55.55	59.87	54.86	56.68	52.94	51.84	57.68	55.42	54.01	40.44	41.8	38.69	1.81
PUFA	**	***	***	**	***	***	**	***	***	**	***	***	**	***	***	**	***	***	1.81

P-Primiparous, S-Secondiparous and PL-Pluriparous. T0-control diet; T1,T2,T3,T4-Experimental times, T5-45 days after the last administration of the enriched feed (end lactation). SEM: standard error of the mean. Analysis Two-way Anova P and S C18:2 n6 T5 vs T0,T1,T2,T3,T4 (p<0.001)***, P C18:1 n9 T5 vs T0,T1,T2,T3,T4 (p<0.001)***, PL C18:1 n9 T0 vs T2 (p<0.001)***; C18:2 n6 T0 vs T2 (p<0.01)**. P (PUFA vs MUFA T0, T1,T2,T3,T4,T5 p<0.01 **), S (PUFA vs MUFA T0,T1,T2,T3,T4,T5 p<0.001 ***), PL (SFA vs MUFA T0, T1,T2,T3,T4,T5 p<0.01**; PUFA vs MUFA T0,T1,T3,T3,T4,T5 p<0.001***).