Figure 7.

Comparison of Na⁺, K⁺, Li⁺, Ca²⁺, and Mg²⁺ Accumulation in Shoots between sas1 and Wild Type.

Twelve-day-old plants grown in vitro were cultivated for 6 additional days on standard medium supplemented with NaCl (Na⁺), KCl (K⁺), CaCl₂ (Ca²⁺), or MgCl₂ (Mg²⁺) at a final concentration of 35 mmol·L⁻¹ or with LiCl (Li⁺) at a final concentration of 2.5 mmol·L⁻¹. For each treatment, the shoot content of the cation that was added to the standard culture medium was determined from a pool of 20 plants. Ion content in sas1 shoots was expressed as percentage of the wild-type value, which was standardized to 100. Wild-type ion contents were 1.6 ± 0.1 mmol·g⁻¹ dry weight for Na⁺, 3.5 ± 0.3 mmol·g⁻¹ dry weight for K⁺, 19.8 ± 1.2 μmol·g⁻¹ dry weight for Li⁺, 0.7 ± 0.1 mmol·g⁻¹ dry weight for Ca²⁺, and 0.8 ± 0.08 mmol·g⁻¹ dry weight for Mg²⁺. The error bars indicate standard deviation; n = 6. WT, wild type.