Table 1.

Na⁺ Accumulation in Calli, Detached Leaf Fragments, and Different Organs of Intact Plants

		Na+ Contentc
Tissuea	Number of Repeatsb	Wild Type	sas1	Ratio of sas1 to Wild Type
A Calli	5	265 ± 63	300 ± 100	1
Leaf fragments	6	1940 ± 190	1960 ± 220	1
B Bud	4	19.9 ± 4.5	187 ± 32	9.5
Flower	4	25.7 ± 5.1	325 ± 20	12.5
Green silique	4	21.0 ± 4.8	234 ± 37	11
Stem	4	90.8 ± 12.2	492 ± 48	5.5
Senescing leaf	4	71.7 ± 7.3	610 ± 48	8.5
Expanding leaf	4	14.2 ± 4.4	326 ± 24	23
Hypocotyl	4	66.4 ± 21.1	171 ± 37	2.5
Root	4	39.3 ± 6.1	45 ± 20	1
C Xylem sap	6	0.9 ± 0.25d	5.1 ± 1.2d	5.5
D Shoot in hydroponic culture	10	923 ± 75	2310 ± 330	2.5
Root in hydroponic culture	10	562 ± 22	186 ± 65	0.35
E Shoot in vitro	5	986 ± 67	2450 ± 140	2.5
Root in vitro	5	354 ± 31	262 ± 18	0.75

^aSix-week-old calli were cultivated for 6 days in liquid medium containing 35 mM NaCl. Leaf fragments collected from 4-week-old greenhouse-cultivated plants were incubated for 1 day in aerated 0.2 mM CaSO₄ and 80 mM NaCl under light at 25°C. Plants cultivated in vitro or in hydroponic culture were grown for 12 or 16 days, respectively, on standard medium and then for an additional 6 days on standard medium supplemented with 35 mM NaCl. The other plant tissues and the xylem sap were collected from 6-week-old mature plants grown in the greenhouse.

^bLeaf fragments were analyzed individually. The other samples were collected from pools of at least six plants for greenhouse-cultivated plants and at least 20 plants for plants cultivated in vitro or hydroponically.

^cNa⁺ content expressed in μmol·g⁻¹ dry weight ±sd.

^dNa⁺ concentration expressed in mmol·L⁻¹.