Figure 7.

Piperidine CD4mc analogs sensitize HIV-1-infected cells to ADCC. Primary CD4 T cells were infected with HIV-1_CH58TF (A,D), HIV-1_JRFL (B,E), or HIV-1_AD8 (C,F). HIV+ plasma (1:1000 diluted) was used for staining (A–C) or ADCC (D–F) in the presence of 50µM different compounds or with an equivalent volume of vehicle (DMSO). An Alexa Fluor 647-conjugated anti-human IgG secondary Ab was then used for fluorescent labeling (A–C). Median fluorescence intensity (MFI) in the presence of compounds or that of DMSO was shown; for ADCC, infected cells were used as target cells in a FACS-based ADCC assay that measures the killing of infected p24+ cells_. The assay determines susceptibility to ADCC mediated by a 1/1000 dilution of plasma from HIV-1-infected individuals in the presence of 50µM different compounds or with an equivalent volume of vehicle (DMSO). (G–I) The correlation between cell-surface staining with HIV+ plasma and ADCC was calculated using the Pearson r rank correlation. Data shown are the mean ± SD of at least three independent experiments (plasma from more than 5 HIV-1-infected individuals). Statistical significance was evaluated using an unpaired t test (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant).