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. 2023 May 17;15(5):1185. doi: 10.3390/v15051185

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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BLI competition assay. (A) BLI measurements of tested CD4mcs competition with sCD4 binding to LMHT gp120_{CRF01_AE} core_e enables determination of the concentration required to outcompete 50% of CD4-Core_e binding (C₅₀). (B) Correlation of ADCC activity against HIV-1_AD8 and C₅₀ from the sCD4 competition assays. (C) Correlation of neutralization of HIV_CH58TF (IC₅₀) and C₅₀ from the sCD4 competition assays.