Table 1.

Benefits and drawbacks of different DNA donor template delivery platforms.

Delivery Platform	Benefits	Drawbacks
Non-viral delivery of naked DNA	Limited DNA damage response (DDR) and/or immune response	ssODNs can only deliver relatively short sequences, thus cannot mediate gene correction via introducing a normal copy of cDNA or replacing large segments of the gene (ssDNA with Cas9 target sites could allow for introduction of larger donor sequences)
	Relatively simple to synthesize	Cannot always enable reporter gene cassettes
		Degraded quickly upon entry to cells
AdV	Superior carrying capacity (~35 kb)	Reduced HDR efficiency
	Markedly reduced extent of HDR-independent insertion into the genome due to the capped ends
AAV	Show the highest HDR efficiencies among the currently known vectors.	Triggers a DDR proportional to the MOI used
		Limited carrying capacity (~4.8 kb)
		Can lead to unwanted off-target donor integration
IDLV	Show marked HDR efficiency	Have not been widely applied or accepted yet; and follow-up studies are being conducted to elucidate the potential benefits
	Relatively large carrying capacity (~10 kb)
	Triggers a smaller DDR than AAV vectors
	Less unwanted off-target integration