Table 1.

Summary of selected liquid chromatography and mass spectrometry conditions.

Mobile phase.	Binary system of 0.1% Formic acid in H2O (A) and ACN (B)		ESI source	Positive ESI
				High purity N2 gas Drying gas at 10 L/min with pressure (60 psi)
	0.4 mL/min.
	Elution time: 45 min.
Agilent Zorbax eclipse plus C18 Column	Length	150 mm		ESI temperature: 350 °C
	ID	4.6 mm		Capillary voltage: 4000 V
	Particle size	3.5 μm	Modes	Mass scan, MS2 and MS3
	Temperature	22 ± 1 °C	Collision gas	High purity N2
Gradient elution system	Time in min.	% ACN	Analytes	FBB and its metabolites
	0	5	Mass parameters	Fragmentor voltage (FV): 145 V Amplitude: 1.25 V
	20	60
	25	65
	30	65
	45	5