Figure 6.

Differential Inhibition of Systemic Wound Response Genes by DPI.

Young excised tomato plants with two expanding leaves and a young growing leaf were supplied with phosphate buffer alone or with 100 μM DPI for 0.5 hr, wounded on the lower leaf at time 0, transferred to water, and assayed by RNA gel blotting at the times indicated (see Methods). 18S-rRNA, rRNA (loading control); AOS, allene oxide oxidase; CDI, aspartic proteinase inhibitor; CPI, metallocarboxypeptidase inhibitor; Inh I, proteinase inhibitor I; Inh II, proteinase inhibitor II; LOX, lipoxygenase; PGcat, leaf polygalacturonase catalytic subunit; PPO, polyphenol oxidase; ProSYS, prosystemin.