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. 2023 Apr 25;15(5):1050. doi: 10.3390/v15051050

Figure 2.

Figure 2

Allicin exhibits inhibitory effects on multiple stages of the PRRSV life cycle. A dose of 1 MOI TA-Gluc2 (A) or NL1207 (B) pre-incubated with allicin at different concentrations (0, 10, 20, 40, 80, 100, 200 μg/mL) at 37 °C for 2  h was used to infect MARC-145 cells. At 24 hpi, culture supernatants were harvested for quantification of virus progeny by qRT-PCR targeting PRRSV ORF6. PRRSV infection was also monitored by IFA detection of PRRSV N in cells infected with TA-Gluc2 (C) or NL1207 (D). (E) A schematic diagram of the time course study of allicin’s inhibitory effect on PRRSV infection. (F) A time-of-drug addition assay. MARC-145 cells were infected with PRRSV TA-Gluc2 at an MOI of 1, and the culture supernatants were replaced with fresh medium supplemented with 100  μg/mL allicin or 0.1% DMSO at 2, 4, and 6 hpi. At 24 hpi, culture supernatants were collected for the quantification of virus progeny by qRT-PCR targeting PRRSV ORF6. **, p-value < 0.01; ns, not statistically significant.