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. 2023 Apr 25;15(5):1050. doi: 10.3390/v15051050

Figure 4.

Figure 4

Allicin inhibits viral replication during PRRSV infection. (A) A schematic diagram of the replication assay. (B) The cytotoxicity effect of allicin on BHK-21 cells. BHK-21 cells were treated with allicin at various doses ranging from 0 μg/mL to 100 μg/mL for 24 h. Then, the cytotoxicity of allicin was determined using a CCK-8 assay. BHK-21 cells in 24-well plates were transfected with the infectious cDNA clone pCMV-TA-Gluc2 at 1 µg/well. At 4 hpt, cells were treated with allicin at different concentrations (0, 10, 20, or 40 μg/mL). At 24 hpt, culture supernatants were harvested for the gaussia luciferase assay and viral RNA extraction. The viral loads in the culture supernatants were determined by the expression levels of gaussia luciferase (C) and a qRT-PCR targeting PRRSV ORF6 (D). ***, p-value < 0.001; ****, p-value < 0.0001.