Figure 4.

CNS Titers in C57BL/6 mice infected with different CHIKV strains via different infection routes. (A–C) Mice were infected IC with 1000 PFU (A,B) or IN with 105 PFU (C) of SINV TE or CHIKV SL15649, AF15561, or SM2013, and titers were performed by plaque assay in brains (A,C) and spinal cords (B) collected at 1, 3, 5, or 7 DPI. (D) Mice were infected with 1000 PFU of SINV TE or CHIKV SL15649, AF15561, or SM2013 by subcutaneous footpad, and titers were performed by plaque assay in ipsilateral feet (closed symbols) and brains (open symbols) collected at 5 DPI; dotted horizontal lines represent assay limit of detection; for (A), “Infect” tick mark on y-axis represents input virus amount; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, Tukey’s multiple comparisons.