Figure 6.

CD4+ and CD8+ T cell infiltration to the corpus callosum of B6 mice following CHIKV infection. Four-to-six-week-old B6 mice were infected IC with CHIKV SL15649, AF15561, or SM2013 or mock infected with PBS, and brains were collected at 7 DPI for co-staining for CD4+ and CD8+ T cells by IHC. (A) Representative 200× photomicrographs of the corpus callosum area of B6 mouse brains showing CD4+ (brown) and CD8+ (bright purple) T cells. CD4+ T cells (B) and CD8+ T cells (C) were quantified in the corpus callosum, and the ratio of CD4:CD8 was calculated for each mouse (D). For (D), dotted horizontal line represents an equal ratio of CD4+ to CD8+ T cells; * p < 0.05; ** p < 0.01; Dunn’s multiple comparisons tests.