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. 2023 May 22;15(10):2408. doi: 10.3390/polym15102408

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Hep G2 systemic cytotoxicity co-culture model. (a) Co-culture model membrane stability in the percentage of the initial TEER value. * Represents the statistically significant differences (p < 0.05) found between conditions in each sampling point. (b) Cytotoxicity profile of the evaluated samples against Hep G2 in the basolateral side of the co-culture model. The dotted line represents the 30% cytotoxicity limit as defined by the ISO 10993-5:2009 standard (ISO, 2009). Different letters represent the statistically significant differences (p < 0.05) found for each condition between sampling times. * Represents the statistically significant differences (p < 0.05) found between the test conditions and the blank (media) control.