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. 2023 May 17;16(5):758. doi: 10.3390/ph16050758

Figure 4.

Figure 4

Compound 10b regulated expression of FXR downstream genes. (A) Relative mRNA level of SHP; (B) Relative mRNA level of BSEP; (C) Relative mRNA level of CYP7A1; (D) Relative mRNA level of SREBP1c; (E) Relative mRNA level of PEPCK; (F) Expression level of G6Pase. HepG2 cells were seeded in a 6-well plate and treated with DMSO and varying concentrations of compound 10b for 24 h with or without 50 μM of 1. The mRNA levels of SHP, BSEP, CYP7A1, SREBP1c, PEPCK, and G6Pase were quantified and normalized to β-actin expression. The results, expressed as mean ± SE of three independent experiments, indicate statistical significance as *: p < 0.05, **: p < 0.01, and ***: p < 0.001 vs. control and p < 0.01, and ##: p < 0.01, ###: p < 0.001 vs. 1 (50 μM).