Figure 2.

ADE activity of the ZV54 mAb glycovariants for DENV and ZIKV infection. DENV-2 (A) or ZIKV (B) samples were mixed with serial dilutions of the ZV54 mAb glycovariants or a generic IgG negative control and then incubated with FcγRIIa⁺ K562 cells. The cells were then washed, fixed, permeabilized, and stained with an anti-flavivirus E antibody. The DENV-2 or ZIKV infected cells were identified by flow cytometry. The experiments were performed at least twice, with technical triplicates for each sample of the presented results (means ± SDs).