Table 1.
N-glycosylation profile of the ZV54 mAb variants. Purified ZV54CHO, ZV54WT, and ZV54ΔXF were separated by SDS-PAGE and their heavy chains were excised, trypsin-digested, and analyzed by LC-ESI-MS. The percentage of each N-glycoform was assigned based on the approximate molar ratio of the height of the corresponding glycopeptide peak. The glycoforms were annotated based on the nomenclature of the Consortium for Functional Glycomics.
| Major N-Glycan Species (in %) | Schematic Presentation | ZV54CHO (%) | ZV54WT (%) | ZV54ΔXF (%) |
|---|---|---|---|---|
| GnGn |
|
100 | ||
| GnGnXF3 |
|
100 | ||
| GnGnF6 |
|
68 | ||
| MGnF6 |
|
6 | ||
| AGnF6 |
|
22 | ||
| AAF6 |
|
4 |
: Mannose, 
: N-acetylglucosamine, 
: Fucose, 
: Galactose, 
: Xylose.