Table 1.
O-Methyltransferase Activities and Lignin Content and Composition in Transgenic Alfalfa with Modified Expression of COMT and CCOMT
| Line | COMT (pkat/mg) |
CCOMT (pkat/mg) |
Acetyl Bromide Lignin (% Dry Wta) |
Klason Lignin (% Dry Wt) |
S Lignin (μmol/g Dry Wt) |
G Lignin (μmol/g Dry Wt) |
5-Hydroxy G Lignin (μmol/g Dry Wt) |
S/G Ratio |
|---|---|---|---|---|---|---|---|---|
| Control | ||||||||
| 1 | 5.98 | 22.35 | 16.07 | 17.21 | 158.8 | 305.4 | 0 | 0.52 |
| 2 | 6.55 | 23.77 | 17.79 | 17.91 | 152.8 | 325.2 | 0 | 0.47 |
| 48 | 8.19 | 21.13 | 17.52 | 17.64 | 156.2 | 279.4 | 0 | 0.56 |
| COMT silenced | ||||||||
| SC4 | 1.06 | 20.11 | 16.48 | 12.67 | 10.4 | 227.9 | 7.4 | 0.05 |
| SC5 | 1.24 | 22.26 | 16.35 | 12.46 | 8.6 | 246.1 | 8.8 | 0.04 |
| SC52 | 1.19 | 22.36 | 16.83 | 14.15 | 17.1 | 223.7 | 1.8 | 0.07 |
| COMT antisense | ||||||||
| AC310 | 0.31 | 22.17 | 16.97 | 15.30 | 0 | 248.2 | 0 | 0 |
| CCOMT antisense | ||||||||
| ACC305 | 14.39 | 0.78 | 16.36 | 14.58 | 159.1 | 150.2 | 0 | 1.05 |
| ACC315 | 8.06 | 1.07 | 15.31 | 15.50 | 164.0 | 243.6 | 0 | 0.69 |
| COMT/CCOMT silenced | ||||||||
| DS14 | 0.78 | 5.59 | 16.54 | 14.72 | 54.0 | 223.7 | 0 | 0.23 |
| COMT/CCOMT antisense | ||||||||
| DA302 | 0.81 | 1.15 | 16.78 | 15.06 | 14.0 | 303.0 | 0 | 0.05 |
a
Wt, weight.