TABLE 2.
Kinetic parameters for the reduction of 4H2B by purified enzymes.
Enzyme | K m | k cat | k cat / K m |
---|---|---|---|
[mM] | [min−1] | [M−1·min−1] | |
LnRCR | 28.0 ± 3 | 1.28 × 104 | 4.6 × 105 |
LnRCRSer154Lys | 20.1 ± 2 | 1.98 × 104 | 9.9 × 105 |
LnRCRSer154Glu | 30.1 ± 4 | 1.30 × 104 | 3.3 × 105 |
LnRCRSer154Asp | 35.6 ± 2 | 1.33 × 104 | 3.7 × 105 |