Fig. 7.

bNAb-Fab binding to BG505 and B41 SOSIP.664 SOSIP,664. Fabs of PTG145 or PGT151 were injected over trimer immobilized freshly in each cycle at concentrations as indicated in the color-coded legend. BG505 SOSIP.664 trimer was 2G12-purifed and B41 SOSIP.664 trimer 2G12- or PGT145-purified, as indicated. Each sensorgram shows the response after background subtraction on the y axis (response units, RU) over time after start of injection on the axis (s). Association was monitored for 300 s and dissociation for 600 s. The model-fitted functions (heterogeneous-ligand for BG505 and Langmuir for B41) are shown as thin black curves. The sensorgram for PGT151 Fab binding to PGT145-purified B41 trimer has no model curves because no model fitted to the weak binding. The number of replicate titrations is given in Table 1