Table 1.

Langmuir modeling^a

SOSIP.664 trimer	Fab	Affinity purification	konb (1/Ms)	koffb (1/s)	KDb (nM)	Smb
BG505	PGT145	2G12 (n = 3)	1.8 × 104 ± 3.7 × 102	5.1 × 10–4 ± 2.0 × 10–6	29 ± 0.69	0.74 ± 6.8 × 10–3
	PGT151	2G12 (n = 2)	8.7 × 104 ± 3.5 × 102	1.8 × 10–4 ± 5.0 × 10–7	2.0 ± 1.3 × 10–2	1.9 ± 7.5 × 10–3
B41	PGT145	2G12 (n = 3)	2.0 × 104 ± 8.8 × 102	1.2 × 10–4 ± 1.5 × 10–5	6.2 ± 0.28	0.63 ± 1.6 × 10–2
		PGT145 (n = 3)	3.2 × 104 ± 2.3 × 103	< 10–5	< 0.5	0.87 ± 4.8 × 10–2
	PGT151	2G12 (n = 3)	4.0 × 104 ± 4.1 × 103	3.1 × 10–3 ± 2.4 × 10–4	77 ± 5.0	0.45 ± 1.5 × 10–2
		PGT145 (n = 2)	Minimal binding: < 10 RU

^aTabulated values are means ± S.E.M of n independent replicates

^bk_on and k_off are the on- and off- rate constants, respectively; K_D is equilibrium dissociation constant = k_off /k_on. S_m denotes the stoichiometry, i.e., number of Fab molecules per trimer