Table 2.

Heterogeneous-ligand modeling^a

SOSIP.664 trimer	Fab	Affinity purification	kon1b (1/Ms)	koff1b (1/s)	kon2b (1/Ms)	koff2b (1/s)	KD1b (nM)	KD2b (nM)	Sm1b	Sm2b	Sm1b + Sm2b
BG505	PGT145	2G12 (n = 3)	2.9 × 104 ± 7.5 × 102	< 10–5	5.0 × 103 ± 7.0 × 102	2.0 × 10–3 ±  .1 × 10–5	< 0.5	4.1 × 102  ± 64	0.46 ± 1.6 × 10–2	0.46  ± 2.4 × 10–2	0.92  ± 3.8 × 10–2
	PGT151	2G12 (n = 2)	1.4 × 105 ± 1.3 × 103	< 10–5	2.8 × 104 ± 1.0 × 103	7.6 × 10–4 ±  .5 × 10–5	< 0.1	27 ± 1.9	1.2 ± 1.9 × 10–2	0.70  ± 1.1 × 10–2	1.9 ± 7.7 × 10–3
B41	PGT145	2G12 (n = 3)	5.2 × 104 ± 1.4 × 104	1.9 × 10–4 ± 3.6 × 10–5	1.6 × 104 ± 1.5 × 103	1.1 × 10–4 ±  .7 × 10–5	3.7 ± 0.23	6.6 ± 0.80	4.4 × 10–2 ±  .4 × 10–2	0.59 ± 3.6 × 10–2	0.63 ± 1.8 × 10–2
		PGT145 (n = 3)	3.5 × 104 ± 1.4 × 104	< 10–5	4.5 × 104 ±  1.1 × 104	< 10–5	< 0.5	< 0.5	0.13 ± 0.13	0.74 ± 0.16	0.87 ± 5.1 × 10–2
	PGT151	2G12 (n = 2)	3.1 × 104 ± 1.9 × 103	8.5 × 10–4 ±  2.5 × 10–4	2.5 × 104 ±  5.5 × 103	7.8 × 10–4 ±  .8 × 10–5	28 ± 9.8	34 ± 12	0.45 ± 2.9 × 10–2	4.1 × 10–3  ± 4.0 × 10–3	0.45 ± 2.5 × 10–2

^aTabulated values are means ± S.E.M of n independent replicates

^bThe constants and S_m are explained in Table 1 but here heterogeneous model allocates distinct values for two different sites, each indicated by the subscript 1 or 2. S_{m1 +} S_m2 = the cumulative stoichiometry