Figure 6.

Effect of CSF co-incubation from L. casei IMAU60214 on the cytotoxic damage induced with the DEC strains on HT-29 human epithelial cells. (A) Quantification of cell damage by MTT reduction. (B) Representative images of the structural analysis by visualization in phase contrast microscopy using an inverted microscope with a 20× dry objective in the absence of CSF and with CSF (80%). The magnification of these picture is 400×. The values represent the mean ± standard deviation of three independent bioassays for each of the pathogenic strains. * significant difference among the treatments (p > 0.05).