Table 1.
Characterization of AvrPto Mutants Disrupting the Pto Interaction
| Mutanta | Pto Interactionb | Protein Accumulationc |
|---|---|---|
| AvrPto | Yes | Normal |
| M11T | No | Low |
| L43P | No | Normal |
| S46P | No | Low |
| L72P | No | Normal |
| Q86R | No | Normal |
| S94P | No | Normal |
| I96T | No | Normal |
| G99V | No | Normal |
| N105K | No | Normal |
a
Mutants were named according to their amino acid substitutions. Single-letter code is used to designate the amino acids.
b
Pto interaction was determined by yeast two-hybrid assay.
c
Mutant proteins were examined by protein gel blot analysis using the anti-AvrPto antibodies.