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. 2023 May 4;13(5):627. doi: 10.3390/metabo13050627

Figure 7.

Figure 7

Mig6 controls EGF, but neither IGF1 nor HGF, pro-survival signaling pathways. (A–F) 832/13 cells were transduced with adenoviruses carrying cmvGFP vs. cmvMig6 or siCon vs. siMig6. Post transduction, cells were starved in 5 mM glucose and 0.1% BSA medium for 2 h, followed by 10 ng/mL recombinant rat EGF stimulation for 5 min. (G,H) After adenoviral transduction and starvation as in ((A,D), 832/13 cells were treated with recombinant human HGF or IGF-1 for 5 min. Protein levels of p-EGFR, p-Erk, Erk, p-Akt, Akt, and tubulin were analyzed by immunoblotting. Groups were compared using ANOVA with Bonferroni post hoc tests. n ≥ 3. * p < 0.05 vs. non-stimulated. # p < 0.05 vs. control virus, stimulated condition.