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. 2023 May 4;13(5):627. doi: 10.3390/metabo13050627

Figure 8.

Figure 8

Mig6 suppression dampens apoptosis during glucolipotoxicity. (A) 832/13 cells were transduced with adenoviral vectors carrying either a scrambled control siRNA (siCon) or shRNA sequence against Mig6 (siMig6). Mig6 mRNA levels were determined by qRT-PCR. Groups were compared using Student’s t-test. n = 4; * p < 0.05. (B–D) Transduced cells were treated with GLT and EGF, as described previously. Protein levels of p-EGFR, EGFR, p-Erk, and Erk were determined by immunoblotting. Data are reported as fold induction related to the GLT-treated, non-EGF-stimulated group. Groups were compared using ANOVA with Bonferroni post hoc tests. n ≥ 3. * p < 0.05 vs. EGF-treated. # p < 0.05 vs. siCon EGF-stimulated. (E) Caspase 3/7 activity was measured following exposure to glucolipotoxic conditions in 832/13 cells siCon or siMig6. Groups were compared using ANOVA with Bonferroni post hoc tests. n = 3 experiments; * p < 0.05 vs. siCon.