Table 1.
Name | Vendor, Model Number | Dilution |
---|---|---|
Anti-Actin | MP Biomedicals, #691002 | 1:5000 |
Anti-Akt | Cell Signaling, #2920 | 1:1000 |
Anti-caspase 3 | Cell Signaling, #9662 | 1:1000 |
Anti-CHOP | Santa Cruz, #7351 | 1:250 |
Anti-EGFR | Sigma-Aldrich, #E3138 | 1:1000 |
Anti-eIF2α | Cell Signaling, #5324 | 1:1000 |
Anti-ERK1/2 | Cell Signaling, #4696 | 1:1000 |
Anti-γ-tubulin | Sigma-Aldrich, #T6557 | 1:5000 |
Anti-GAPDH | Abcam, #Ab9483 | 1:5000 |
Anti-Mig6 | Santa Cruz, #D-1 | 1:250 |
Anti-phospho-Akt (Thr308) | Cell Signaling, #4056 | 1:1000 |
Anti-phospho-EGFR (Tyr1068) | Cell Signaling, #3777 | 1:1250 |
Anti-phospho-eIF2α (Ser51) | Cell Signaling, #3398 | 1:1000 |
Anti-phospho-ERK1/2 (Thr202/Tyr204) | Cell Signaling, #4370 | 1:2000 |
IRDye 800 or 700 fluorophore-conjugated antibodies | LI-COR | 1:10,000 |